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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6132
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pubmed:dateCreated |
1987-9-23
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pubmed:abstractText |
The genome of the human immunodeficiency virus (HIV) contains several open reading frames (ORFs) not present in other viruses. The 'A' gene, also known as Q2 P'3, ORF-1(4) or sor5, partially overlaps the pol gene; its protein product has a relative molecular mass of 23,000 (Mr 23K) and is present in productively infected cells. The function of this protein is unclear; mutant viruses deleted in 'A' replicate in and kill CD4+ lymphocyte lines, but the high degree of conservation of the deduced amino-acid sequence in nine different HIV isolates (80%) and the presence of analogous genes in HIV-2 and other lentiviruses suggest that the gene function is an important one. Here we describe a mutant virus deficient in the 'A' gene which produces virion particles normally; however, the particles are approximately 1,000 times less infective than wild type. Transcomplementation experiments partially restore infectivity. The mutant virus spreads efficiently when virus-producing cells are co-cultivated with CD4+ lymphocytes, however, indicating that HIV can spread from cell to cell in a mechanism that does not require the 'A' gene product and probably does not require the production of infective virus particles.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0028-0836
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
328
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
728-30
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2441266-Amino Acid Sequence,
pubmed-meshheading:2441266-Cell Communication,
pubmed-meshheading:2441266-Chromosome Deletion,
pubmed-meshheading:2441266-Cloning, Molecular,
pubmed-meshheading:2441266-Colonic Neoplasms,
pubmed-meshheading:2441266-Genetic Complementation Test,
pubmed-meshheading:2441266-HIV,
pubmed-meshheading:2441266-Humans,
pubmed-meshheading:2441266-Mutation,
pubmed-meshheading:2441266-Phenotype,
pubmed-meshheading:2441266-RNA-Directed DNA Polymerase,
pubmed-meshheading:2441266-Recombination, Genetic,
pubmed-meshheading:2441266-Transfection,
pubmed-meshheading:2441266-Virion
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pubmed:articleTitle |
The HIV 'A' (sor) gene product is essential for virus infectivity.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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