Linked Life Data

2441250

Source:http://linkedlifedata.com/resource/pubmed/id/2441250

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1987-9-1
pubmed:abstractText
Twenty-seven murine monoclonal anti-hen egg-white lysozyme (HEL) antibodies were tested with a panel of nine antigens; eight avian lysozymes and human lysozyme. The antibodies were arranged into 10 groups based on their antigen specificity and cross-reactivity. Antigenic determinants recognized by each group of antibodies were tentatively identified. They are located at different points of the HEL accessible surface in agreement with the notion that its entire surface has an antigenic potential. The affinity constants of antibodies representative of seven of the 10 groups range from 0.79 X 10(7) to 5.3 X 10(7) M-1. Heteroclitic antibodies occurring in some of the groups bind heterologous lysozymes with somewhat higher association constants than those for the homologous antigen (HEL). Their broader specificities do not correlate with overall lower association constants, but rather with the occurrence of public epitopes in the panel of avian lysozymes. Although the reaction of two antibodies with the solid-phase coupled antigen is not always additive, detailed interpretation of these results and the observation of actual ternary complexes Fab-HEL-Fab rules out the occurrence of conformational changes in the complexed antigen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0161-5890
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
97-108
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Antigen specificity and cross-reactivity of monoclonal anti-lysozyme antibodies.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't