Linked Life Data

2438235

Source:http://linkedlifedata.com/resource/pubmed/id/2438235

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1987-7-23
pubmed:abstractText
Three MAbs endowed with comparable affinity for 3 distinct epitopes of a HMW-MAA have been employed to study whether the use of combinations of MAbs might improve the sensitivity of methods for the quantitation of TAAs both on the cell surface and in soluble form. To this end, different combinations and sequences of application of the 3 MAbs were used both in cell-surface binding experiments and in solid-phase radioimmunoassays. Results of this study show that only selected combinations of MAbs are capable of significantly improving the sensitivity of methods for HMW-MAA detection, and that such improvements depend on the order and mode of addition of the 3 reagents. Thus, mixtures of MAbs to distinct epitopes carried by a single TAA molecule are likely to increase the sensitivity of tests for tumor detection both in vitro (i.e. radioimmunoassays) and in vivo (i.e. immunoscintigraphy). However, because the binding properties of such mixtures do not simply reflect those of the individual reagents, protocols for the use of MAb cocktails should be critically evaluated before clinical use.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0020-7136
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
729-36
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Multiple epitope recognition: an approach to improved radioimmunodetection of tumor-associated antigens.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't