Linked Life Data

2438142

Source:http://linkedlifedata.com/resource/pubmed/id/2438142

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-7-23
pubmed:abstractText
The antigen fine specificity of T cell hybridomas recognizing the horse apocytochrome c fragment 1-65, restricted to the I-Ab molecule, was determined to gain some insight into the molecular nature of T cell antigenic peptides. Two major groups of clones specific for distinct subsites, namely residues 1-38 and 39-65, could be identified. Hybridomas recognizing the latter determinant were further tested with different horse cytochrome c peptides and analogues. This analysis revealed the presence of at least two epitopes encompassed by residues 47-53 and 48-53. Furthermore, clones specific for the amino acid sequence 48-53 showed considerable heterogeneity in respect to the antigen concentration required to obtain 50% of the maximal interleukin 2 secretion. Most prominent was the heteroclitic response towards tuna cytochrome c which differs at positions 44, 46 and 47 from the horse cytochrome c molecule in the relevant region. Comparison of the conformation of the sequence 43-46 between the two cytochrome c suggests that this segment, which forms a 3(10) bend, may be important in maintaining the proper structure of the antigenic determinant. Moreover, the variations up to 180-fold in the concentrations of the cross-reacting cytochrome c and peptides required for stimulation were not always correlated with the maximal interleukin 2 secretion they induced. This indicates that the biological response, that is supposed to be an indication of the affinity of the T cell receptor for its ligand, is not necessarily a function of the antigen concentration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
651-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Multiple T cell antigenic determinants identified within a limited region of the horse cytochrome c molecule.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't