Linked Life Data

2438049

Source:http://linkedlifedata.com/resource/pubmed/id/2438049

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-7-9
pubmed:databankReference
pubmed:abstractText
The mitochondrial DNA of trypanosomes is organized as a network of catenated circular DNA molecules called the kinetoplast. The minicircles of the kinetoplast are 1 kb circular DNA molecules present at 5,000-10,000 copies per network. The maxicircles are 20 kb circular molecules present at 50-100 copies per network. Maxicircles are transcribed and are thus analogous to mitochondrial DNAs. Here we show that, contrary to previous reports, the minicircles of T. brucei are also transcribed. A minicircle transcript of approximately 240 nucleotides is present in bloodstream and insect developmental stages of the parasite, is enriched in purified mitochondrial preparations, and is efficiently synthesized in vitro. The minicircle cDNA overlaps the conserved region of the T. brucei minicircle and is juxtaposed to a 12 base sequence common to all minicircles. These findings indicate that minicircles, in addition to their previously proposed structural role, are transcribed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
625-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Transcription of kinetoplast DNA minicircles.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.