Linked Life Data

2437061

Source:http://linkedlifedata.com/resource/pubmed/id/2437061

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1987-6-10
pubmed:abstractText
Sertoli cell monolayers were prepared from 30-day-old rat testes and cultured for 7 days to eliminate contaminant germ cells. Some of these monolayers were co-cultured with a spermatogenic cell preparation enriched in pachytene spermatocytes and round spermatids (fraction 3 from a Percoll gradient), isolated from 30-day-old rat testes. After co-culture for 4 to 48 h, germ cells were removed. RNA synthetic activity in rat Sertoli cell cultures alone was 216,800 +/- 66,480 dpm [3H]uridine.2h-1 X 10(6) cells-1 (mean +/- SD) compared to 98,390 +/- 23,595 in rat Sertoli cells which had been co-cultured with germ cells of fraction 3 for 24 h (P less than 0.01). By contrast, RNA synthesis in Sertoli cell monolayers prepared from immature pigs were unaffected by co-culture with rat germ cells. A similar inhibitory effect of germ cells was observed in rat Sertoli cells stimulated with FSH or testosterone. Culture medium, conditioned by 20 h culture of a fresh preparation of rat spermatogenic cells of fraction 3, was active in inducing the inhibitory effect on RNA synthesis in rat Sertoli cells. Co-culture of rat Sertoli cells with germ cells of this fraction also decreased the incorporative of [3H]thymidine into DNA in rat Sertoli cells, from 9061 +/- 3339 to 4766 +/- 526 dpm.2h-1 X 10(6) cells-1 (P less than 0.01), but no such change was found in pig Sertoli cells. A different spermatogenic cell preparation, partially deprived of pachytene spermatocytes (fraction 5), stimulated rat Sertoli cell DNA synthesis (Sertoli alone 7833 +/- 2550, Sertoli cells which had been in co-culture with germ cells of fraction 5, 13,300 +/- 2279 dpm.2h-1 X 10(6) cells-1, P less than 0.05). These inhibitory actions of some germ cells on Sertoli cells were observed together with the previously reported simultaneous stimulatory effect of Sertoli cells on germ cells. These Sertoli cell-germ cell interactions of detected in culture may represent regulatory influences operating in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0105-6263
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
424-34
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Inhibition of RNA and DNA synthesis in Sertoli cells by co-culture with spermatogenic cells.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't