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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1987-6-5
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pubmed:abstractText |
A series of four expression plasmids coding for fusion proteins containing foot-and-mouth disease virus (FMDV) sequences was constructed. The fusion proteins contain a large part of beta-galactosidase from Escherichia coli preceded (N-terminal) by 1, 2, 4 or 8 repeats of the antigenic determinant of FMDV consisting of amino acids 137-162 of the capsid polypeptide VP1. All four fusion proteins were efficiently produced in E. coli host bacteria. Immunization of rabbits resulted in FMDV-specific, neutralizing antibodies, the response being dependent on the number of repeats. With enzyme-linked immunosorbent-assay techniques it was shown that the FMDV antigenic determinants are exposed on the surface of the fusion proteins under non-denaturing conditions.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:issn |
0378-1119
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
189-97
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2436976-Animals,
pubmed-meshheading:2436976-Antibodies, Viral,
pubmed-meshheading:2436976-Antigens, Viral,
pubmed-meshheading:2436976-Aphthovirus,
pubmed-meshheading:2436976-Capsid,
pubmed-meshheading:2436976-Epitopes,
pubmed-meshheading:2436976-Escherichia coli,
pubmed-meshheading:2436976-Rabbits,
pubmed-meshheading:2436976-Recombinant Fusion Proteins,
pubmed-meshheading:2436976-Recombinant Proteins
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pubmed:year |
1986
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pubmed:articleTitle |
Synthesis of fusion proteins with multiple copies of an antigenic determinant of foot-and-mouth disease virus.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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