Linked Life Data

2436575

Source:http://linkedlifedata.com/resource/pubmed/id/2436575

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1987-4-27
pubmed:abstractText
Monoclonal antibodies were prepared against a mixture of purified lysosomal enzymes from Dictyostelium discoideum. Three classes of antibodies were found which recognized distinct antigenic determinants on N-linked oligosaccharides of multiple proteins. The structure of the determinants was studied by competition assays using monosaccharides and oligosaccharide/glycopeptide fractions prepared from one Dictyostelium lysosomal enzyme or other sources. The results of these studies suggest that one class of antibody recognizes an epitope containing residues of Man-6-SO4, another recognizes a domain containing a modified GlcNAc, and the third class recognizes an undefined determinant that involves the oligosaccharide. The three determinants are found on multiple overlapping, but nonidentical sets of glycoproteins. The ability to produce monoclonal antibodies against unusual N-linked oligosaccharides offers a powerful tool which can be used to investigate the occurrence, structure, biosynthesis, and the biological roles of these highly immunogenic saccharides.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:volume
253
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
305-14
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Characterization and distribution of multiple antigens on N-linked oligosaccharides of Dictyostelium discoideum proteins.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't