Linked Life Data

2435681

Source:http://linkedlifedata.com/resource/pubmed/id/2435681

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11-12
pubmed:dateCreated
1987-5-18
pubmed:abstractText
The utility of a lectin from Griffonia simplicifolia (GSA II) for demonstrating glycogen in situ was tested on fixed paraffin-embedded sections of a variety of tissues from rodents and man. The histochemical specificity of GSA II conjugated to horseradish peroxidase (GSA II-HRP) for glycogen was documented by the lability of such staining on adjacent sections treated with either malt diastase or alpha-amylase. In some tissue sites, the lectin-HRP conjugate imparted cytoplasmic staining that was diastase- and amylase-labile but resisted digestion with N-acetylglucosaminidase. Reactivity in the latter sites was attributed to glycogen and occurred in cell types having well-documented glycogen content, including liver hepatocytes, skeletal muscle fibres and polymorphonuclear leukocytes. In other tissue loci, GSA II binding was confined to cell surfaces or intracellular compartments, was not affected by prior diastase or amylase digestion, but was abolished with N-acetylglucosaminidase. Staining in these sites was attributed not to glycogen, but instead to glycoconjugate having sugar chains terminated with N-acetylglucosamine. These findings document the affinity of GSA II for glycogen in situ but do not conflict with the biochemically demonstrated affinity of GSA II for terminal N-acetylglucosamine. The results reported here show that the GSA II-HRP method may be useful for detecting physiological or pathological changes in the glycogen content of cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0018-2214
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
589-96
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:articleTitle
Histochemical detection of glycogen using Griffonia simplicifolia agglutinin II.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.