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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1987-3-31
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pubmed:abstractText |
The occurrence and coexpression of the cytoskeletal proteins vimentin and cytokeratins were studied in malignant mesotheliomas and pulmonary carcinomas. For this purpose a double immunoenzyme staining with monoclonal antibodies was developed which made it possible to visualize vimentin and cytokeratins simultaneously within the same cell. A clear distinction between stromal cells (vimentin only) and tumour cells was also obtained. A total of 12 mesotheliomas (six mixed type and six epithelioid type) and 13 carcinomas (eight adenocarcinomas and five large cell undifferentiated carcinomas) were studied. The results revealed a clear difference between mesotheliomas and adenocarcinomas: 11 of 12 mesotheliomas showed coexpression of vimentin and cytokeratins in at least 50% of the tumour cells, while in seven of the eight adenocarcinomas none or only a few cells could be seen with this coexpression. In the undifferentiated large cell carcinomas three of five expressed both components, but in less than 25% of the cells. It is concluded that a reliable double immunoenzyme staining of vimentin and cytokeratins can be used as an additional means to distinguish malignant mesothelioma from pulmonary adenocarcinoma.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0340-6075
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
55-65
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2435052-Adenocarcinoma,
pubmed-meshheading:2435052-Antibodies, Monoclonal,
pubmed-meshheading:2435052-Diagnosis, Differential,
pubmed-meshheading:2435052-Humans,
pubmed-meshheading:2435052-Immunoenzyme Techniques,
pubmed-meshheading:2435052-Keratins,
pubmed-meshheading:2435052-Lung Neoplasms,
pubmed-meshheading:2435052-Mesothelioma,
pubmed-meshheading:2435052-Peritoneal Neoplasms,
pubmed-meshheading:2435052-Pleural Neoplasms,
pubmed-meshheading:2435052-Staining and Labeling,
pubmed-meshheading:2435052-Vimentin
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pubmed:year |
1986
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pubmed:articleTitle |
Simultaneous immunoenzyme staining of vimentin and cytokeratins with monoclonal antibodies as an aid in the differential diagnosis of malignant mesothelioma from pulmonary adenocarcinoma.
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pubmed:publicationType |
Journal Article
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