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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1987-4-8
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pubmed:abstractText |
Schwann cell proliferation induced by a myelin-enriched fraction was examined in vitro. Although nearly all the Schwann cells contained material that was recognized by antisera to myelin basic protein after 24 h, only 1% of the cells were synthesizing DNA. 72 h after the addition of the mitogen a maximum of 10% of the cells incorporated [3H]thymidine. If the cultures were treated with the myelin-enriched fraction for 24 h and then washed, the number of proliferating Schwann cells decreased by 75% when compared with those cells that were incubated with the mitogen continuously. When Schwann cells were labeled with [14C]thymidine followed by a pulse of [3H]thymidine 24 h later, every Schwann cell labeled with [3H]thymidine was also labeled with [14C]thymidine. Although almost every Schwann cell can metabolize the myelin membranes within 24 h of exposure, a small population of cell initially utilizes the myelin as a mitogen, and this population continues to divide only if myelin is present in the extracellular media. The percentage of the Schwann cells that initially recognize the myelin-enriched fraction as a mitogen is dependent upon the age of the animal from which the cells were prepared.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-215315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-371755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-3934342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-5414369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-6046571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-6244318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-6501427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-6512566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-6883129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-7055726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-7085777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-7358790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2434514-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
655-60
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2434514-Animals,
pubmed-meshheading:2434514-Carbon Radioisotopes,
pubmed-meshheading:2434514-Cell Division,
pubmed-meshheading:2434514-Cells, Cultured,
pubmed-meshheading:2434514-DNA Replication,
pubmed-meshheading:2434514-Immunoenzyme Techniques,
pubmed-meshheading:2434514-Kinetics,
pubmed-meshheading:2434514-Myelin Basic Proteins,
pubmed-meshheading:2434514-Myelin Sheath,
pubmed-meshheading:2434514-Rats,
pubmed-meshheading:2434514-Schwann Cells,
pubmed-meshheading:2434514-Thymidine,
pubmed-meshheading:2434514-Tritium
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pubmed:year |
1987
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pubmed:articleTitle |
Developmental changes in myelin-induced proliferation of cultured Schwann cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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