Linked Life Data

2432271

Source:http://linkedlifedata.com/resource/pubmed/id/2432271

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 1
pubmed:dateCreated
1987-2-12
pubmed:abstractText
The fluorescence properties of orcein are described in this paper. Under suitable exciting light, acidic solutions of orcein showed emission peaks at 587 nm (low concentration) and at 620 nm (high concentration). In fluorescence microscopy an intense orange-red emission was found in the cytoplasm and nucleoli from acetic orcein squashes of meristematic cells, as well as in the cytoplasm of Ehrlich tumour cells and in chicken erythrocyte nuclei. When semithin sections of Epon-embedded tissues were treated with orcein, a strong fluorescence reaction (orange-red) appeared in starch granules, cell walls, elastin, collagen, reticulin, keratohyalin, chromatin and mucin. Cytofluorometric measurements of orcein-stained chromatin revealed an emission peak at 585 nm with a shoulder at 620 nm. These spectroscopic and microscopical results suggest the possibility of employing orcein as a fluorochrome.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-2720
pubmed:author
pubmed:issnType
Print
pubmed:volume
144
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Fluorescence reactions of orcein: new applications for an old stain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't