| pubmed-article:2432241 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2432241 | lifeskim:mentions | umls-concept:C0013710 | lld:lifeskim |
| pubmed-article:2432241 | lifeskim:mentions | umls-concept:C0018557 | lld:lifeskim |
| pubmed-article:2432241 | lifeskim:mentions | umls-concept:C0521449 | lld:lifeskim |
| pubmed-article:2432241 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:2432241 | lifeskim:mentions | umls-concept:C0444706 | lld:lifeskim |
| pubmed-article:2432241 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:2432241 | lifeskim:mentions | umls-concept:C0013812 | lld:lifeskim |
| pubmed-article:2432241 | lifeskim:mentions | umls-concept:C0332182 | lld:lifeskim |
| pubmed-article:2432241 | pubmed:dateCreated | 1987-2-17 | lld:pubmed |
| pubmed-article:2432241 | pubmed:abstractText | The hamster egg at fertilization shows transient, periodic hyperpolarizing responses due to a Ca2+-activated K+ conductance. The suggested increase of the intracellular Ca2+ concentration ([Ca2+]i) was measured with Ca2+-sensitive microelectrodes in combination with the voltage-clamp technique. Calculated values of the basal [Ca2+]i before fertilization averaged in the range 0.2-0.4 microM. Insemination of eggs induced periodic Ca2+ transients with exact one-to-one correspondence to periodic, Ca2+-activated K+ currents. They were recorded anywhere in the cytoplasm, even far from the site of sperm-egg fusion, indicating that [Ca2+]i increases in the whole egg upon each response. During a series of responses after insemination the increase in [Ca2+]i reached 1-2 microM in the first three responses and the peak value decreased to 0.7-0.8 microM in the later responses. The rise and decay times of each Ca2+ transient were 6.8 +/- 2.8 and 22 +/- 6 s (mean +/- S.D., n = 36), respectively. No significant difference in the amplitude and time course of the Ca2+ transient was detected in relation to the depth of the cytoplasm about 3-30 micron from the surface. The basal [Ca2+]i increased gradually from 0.39 to 0.56 microM (average in fourteen eggs) during the series of Ca2+ transients. The biological significance and mechanism of the periodic increase in [Ca2+]i and the response time of the Ca2+ electrode are discussed. | lld:pubmed |
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| pubmed-article:2432241 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2432241 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2432241 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2432241 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2432241 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:2432241 | pubmed:issn | 0022-3751 | lld:pubmed |
| pubmed-article:2432241 | pubmed:author | pubmed-author:MiyazakiSS | lld:pubmed |
| pubmed-article:2432241 | pubmed:author | pubmed-author:IgusaYY | lld:pubmed |
| pubmed-article:2432241 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2432241 | pubmed:volume | 377 | lld:pubmed |
| pubmed-article:2432241 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2432241 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2432241 | pubmed:pagination | 193-205 | lld:pubmed |
| pubmed-article:2432241 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:2432241 | pubmed:meshHeading | pubmed-meshheading:2432241-... | lld:pubmed |
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| pubmed-article:2432241 | pubmed:meshHeading | pubmed-meshheading:2432241-... | lld:pubmed |
| pubmed-article:2432241 | pubmed:meshHeading | pubmed-meshheading:2432241-... | lld:pubmed |
| pubmed-article:2432241 | pubmed:meshHeading | pubmed-meshheading:2432241-... | lld:pubmed |
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| pubmed-article:2432241 | pubmed:meshHeading | pubmed-meshheading:2432241-... | lld:pubmed |
| pubmed-article:2432241 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:2432241 | pubmed:articleTitle | Periodic increase of cytoplasmic free calcium in fertilized hamster eggs measured with calcium-sensitive electrodes. | lld:pubmed |
| pubmed-article:2432241 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2432241 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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