Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-11-10
pubmed:databankReference
pubmed:abstractText
In Bacillus subtilis, the extracellular enzyme levansurcrase is synthesized in the presence of sucrose. A termination structure between the transcription start site and the structural gene was the apparent site for regulation by sucrose of transcription into the structural gene. Sequence analysis of the sacB leader region from two strains constitutive for levansucrase synthesis showed a single base change in the stem of this termination structure. This single base change also led to the constitutive synthesis of a sacB'-'lacZ fusion, whereas the wild-type sacB'-'lacZ fusion was induced by the addition of sucrose. S1 nuclease mapping of sacB transcripts with probes labeled either within the termination structure or 3' to the termination structure showed that sucrose did not increase the number of transcripts extending into the termination structure; however, sucrose did increase the number of transcripts extending past the termination structure. Two pleiotropic mutations which affect the expression of levansucrase, sacQ36 hyperproducing [sacQ36(Hy)] and sacU32(Hy), were separately introduced into the strain carrying the sacB'-'lacZ fusion. These mutations each increased the expression levels of the sacB'-'lacZ fusion. S1 mapping showed increased levels of transcript initiating at the sacB promoter in strains with the sacQ36(Hy) and sacU32(Hy) mutations. This increased transcription appeared to be independent of the sucrose-regulated transcription termination, suggesting the existence of at least two different mechanisms for the regulation of sacB expression.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-16561900, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-16590310, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-2422155, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-2993818, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-3007431, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-3019840, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-3082852, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-3086292, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-3118760, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-363519, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-3918985, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-4209418, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-4219582, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-4519026, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-4559594, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-4628133, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-4960898, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6090407, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6162838, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6186986, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6246368, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6260747, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6271763, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6310323, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6402497, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6424671, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6427178, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6436812, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6575390, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-6757864, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428811-7007895
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
168
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
380-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.
pubmed:publicationType
Journal Article