Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-11-7
pubmed:abstractText
We used mouse monoclonal antibodies (MAbs) to characterize Neisseria gonorrhoeae lipooligosaccharide (LOS). LOSs that bound two or more MAbs in a solid-phase radioimmunoassay usually bound them to different LOS components, as separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); strains with multiple LOS components on SDS-PAGE usually bound more than one MAb. However, the LOS of some strains bound the same MAb to two LOS components with different relative molecular weights, and some individual LOS components bound more than one MAb. LOSs from different strains bound different amounts of the same MAb at saturation, reflecting differences in the quantitative expression of individual LOS components. Not all components recognized by MAbs were stained by silver after periodate oxidation. Treatment with NaOH variously affected epitopes defined by different MAbs. MAb 3F11 completely inhibited and MAb 2-1-L8 partially inhibited the binding of 125I-labeled 06B4 MAb to WR220 LOS and WR220 outer membranes in competitive binding studies. Other MAbs did not compete with the binding of 125I-labeled 06B4 to either antigen. We conclude that a strain of N. gonorrhoeae elaborates multiple LOSs that can be separated by SDS-PAGE and that are antigenically distinct. Epitope expression within these glycolipids is complex.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-109466, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-1172191, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-218075, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-3920399, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-3934078, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-405320, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-417017, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6153246, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6156828, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6156902, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6174450, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6176137, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6202806, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6206158, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6207109, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6266278, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6282751, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6403466, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6408006, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6409879, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6432693, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-67149, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6798135, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6806319, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6806436, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-6815278, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-7038848, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-714156, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-72735, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-824376, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-825573, http://linkedlifedata.com/resource/pubmed/commentcorrection/2428752-93575
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Antigenic and physical diversity of Neisseria gonorrhoeae lipooligosaccharides.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.