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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1986-8-13
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pubmed:abstractText |
The cell surface phenotype of pluripotent hemopoietic stem cells (CFU-S) and committed progenitors (CFU-C1, CFU-C2, BFU-E) of mouse bone marrow was analyzed with respect to their binding of wheat germ agglutinin (WGA) and two monoclonal antibodies, anti-GM-1.2 and anti-PGP-1. Stained cells were fractionated on the basis of differences in fluorescence and light scatter intensity using a light-activated cell sorter. The 6% of the cells that bound most WGA and that also had a relatively high forward light scatter (FLS) and low perpendicular light scatter (PLS) contained nearly all stem cells (CFU-S) and progenitors. Anti-GM-1.2 stained only mature myeloid cells, not CFU-S or the in vitro colony-forming cells. Anti-PGP-1 stained all bone marrow cells in varying intensities: lymphoid cells were dull, CFU-S were intermediate, CFU-C2 were brighter, and mature myeloid cells very bright. Enrichment of progenitor cells was performed by a two-step sorting procedure. First, the 6% most WGA-binding cells with high FLS and low PLS were sorted out. A 10-15-fold enrichment of progenitors and CFU-S was obtained. Next, these cells were restained with anti-GM-1.2 or anti-PGP-1 and again fractionated on the FACS. The GM-1.2-negative cells were then another four- to sevenfold more enriched for stem cells and progenitors. Of the cells in this fraction, 95% could be assigned to a colony-forming unit. With anti-PGP-1, CFU-C2 could be partly separated from more early cells such as CFU-S and BFU-E.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Gm-3.2 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Isoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lymphocyte Homing,
http://linkedlifedata.com/resource/pubmed/chemical/Wheat Germ Agglutinins,
http://linkedlifedata.com/resource/pubmed/chemical/fluorescein isothiocyanate-wheat...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9541
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
128
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
133-42
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2424920-Animals,
pubmed-meshheading:2424920-Antigens, Surface,
pubmed-meshheading:2424920-Bone Marrow Cells,
pubmed-meshheading:2424920-Cell Separation,
pubmed-meshheading:2424920-Colony-Forming Units Assay,
pubmed-meshheading:2424920-Flow Cytometry,
pubmed-meshheading:2424920-Fluorescein-5-isothiocyanate,
pubmed-meshheading:2424920-Fluoresceins,
pubmed-meshheading:2424920-Hematopoiesis,
pubmed-meshheading:2424920-Hematopoietic Stem Cells,
pubmed-meshheading:2424920-Isoantigens,
pubmed-meshheading:2424920-Lectins,
pubmed-meshheading:2424920-Mice,
pubmed-meshheading:2424920-Mice, Inbred Strains,
pubmed-meshheading:2424920-Receptors, Lymphocyte Homing,
pubmed-meshheading:2424920-Staining and Labeling,
pubmed-meshheading:2424920-Wheat Germ Agglutinins
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pubmed:year |
1986
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pubmed:articleTitle |
A fractionation procedure of mouse bone marrow cells yielding exclusively pluripotent stem cells and committed progenitors.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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