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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1986-7-23
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pubmed:abstractText |
The interaction of [125I]spiperone and [3H]spiperone with CHAPS solubilized preparations of rat striatal tissue has been compared. Only about 15% of [125I]spiperone binding was displaced by sulpiride compared with about 67% of [3H]spiperone binding. In the presence of (+)-butaclamol the displacement of [125I]spiperone was twice that found with sulpiride suggesting an interaction with sites other than D-2 receptors. The specific binding of [125I]spiperone was not saturable within the maximum concentration range that could be employed and its affinity for soluble preparations was far lower than that of [3H]spiperone. Despite its very high specific activity [125I]spiperone offers no advantage over [3H]spiperone in the identification of dopamine receptors in soluble tissue preparations.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-3573
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
406-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2423678-Animals,
pubmed-meshheading:2423678-Butaclamol,
pubmed-meshheading:2423678-Butyrophenones,
pubmed-meshheading:2423678-Cholic Acids,
pubmed-meshheading:2423678-Corpus Striatum,
pubmed-meshheading:2423678-Female,
pubmed-meshheading:2423678-Kinetics,
pubmed-meshheading:2423678-Ligands,
pubmed-meshheading:2423678-Rats,
pubmed-meshheading:2423678-Rats, Inbred Strains,
pubmed-meshheading:2423678-Receptors, Dopamine,
pubmed-meshheading:2423678-Spiperone
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pubmed:year |
1986
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pubmed:articleTitle |
[125I]Spiperone is not a useful ligand for studying the CHAPS solubilized dopamine D-2 receptor from rat striatum.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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