Linked Life Data

2423509

Source:http://linkedlifedata.com/resource/pubmed/id/2423509

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-7-14
pubmed:abstractText
Sarcoplasmic reticulum (SR) vesicles were reconstituted by the salting out method in the presence of excess phospholipids: the lipid-to-protein ratio ranged from 10 to 100. It was found that the reconstituted vesicles could be separated by KC1 density gradient centrifugation into four types: those having both cation and anion channels (CASR), those having only cation channels (CSR), those having only anion channels (ASR), and those having no ion channels (PSR). From the yield of these vesicles, it was estimated that one native SR vesicle contains 19 cation channels and 1.4 anion channels on average; the amount of cation channels is 14 times larger than that of anion channels. Although all vesicles thus prepared are considered to contain the Ca2+-ATPase protein, the PSR vesicles alone did not take up Ca2+, but they did do so in the presence of valinomycin. This result indicates that the Ca2+-ATPase takes up Ca2+ in an electrogenic manner. The electromotive force was estimated to be about 50 mV.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-924X
pubmed:author
pubmed:issnType
Print
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1071-80
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Reconstitution of sarcoplasmic reticulum Ca2+-ATPase vesicles lacking ion channels and demonstration of electrogenicity of Ca2+-pump.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't