Linked Life Data

2423077

Source:http://linkedlifedata.com/resource/pubmed/id/2423077

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-6-6
pubmed:abstractText
Monoclonal antibodies were raised in mouse against purified calf thymus RNA polymerase II. Three stable hybridoma lines were identified and found to secrete IgM type antibodies. The antibody produced by one clone was found to be directed against the large subunits (IIb and IIc) of the enzyme. In an immuno-blot of electrophoretically separated subunits the monoclonal antibody recognised antigenic determinant on the largest polypeptides of several eukaryotic RNA polymerases II tested. In an in vitro transcription system, this antibody is found to inhibit the specific transcription from the adenovirus major late promoter only when the extract is preincubated with the antibody in the absence of DNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
136
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-7
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
The use of monoclonal antibody to study the functional properties of RNA polymerase II subunits.
pubmed:publicationType
Journal Article