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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1986-5-23
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pubmed:abstractText |
We report the production and characterization of a human monoclonal antibody reactive against the major envelope glycoprotein of human T-cell leukemia virus type I (HTLV-I), a virus linked to the etiology of adult T-cell leukemia. We exposed lymph-node cells derived from a patient with adult T-cell leukemia to the Epstein-Barr virus in vitro and obtained a B-cell clone (designated 0.5 alpha) by a limiting dilution technique. The secreted product of 0.5 alpha is a monoclonal antibody (also designated 0.5 alpha; that is IgG1 and has kappa light chains) that binds to the cell membrane of T-cells infected with HTLV-I and lyses them in the presence of complement. The antibody does not react with HTLV-I-negative T cells. In electroblot assays, the monoclonal antibody detects a 46-kDa glycoprotein in disrupted HTLV-I virions and a 34-kDa product following digestion of the viral protein with endoglycosidase F. These molecules have been reported to represent the HTLV-I env gene products. The antibody does not react with HTLV-II and HTLV-III virions. Glycoproteins of 61 and 68 kDa, which are known to be encoded at least in part by the env gene of HTLV-I, are precipitated by the antibody from endogenously radiolabeled HTLV-I-infected HUT 102-B2 and MT-2 cells, respectively. These results suggest that this human monoclonal antibody reacts with an env-encoded glycoprotein of HTLV-I. By using a competition assay with a biotin-labeled 0.5 alpha antibody, we observed that 15 out of 15 patients with adult T-cell leukemia had antibodies that block binding of the 0.5 alpha antibody to HTLV-I virions. This suggests that the antigen detected by 0.5 alpha antibody is a common epitope recognized in HTLV-I-infected individuals in vivo. This antibody, as well as the general strategy for making human monoclonal antibodies reactive against pathogenic retroviruses, may have diagnostic or therapeutic application.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-198669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-2990681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6087548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6095307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6189183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6195529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6200935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6204380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6206569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6224522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6244013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6261256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6264163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6274993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6275274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6320527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6324349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6327819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6328528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6505692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6582477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6600519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6601276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-6981847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2422659-7061867
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2672-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2422659-Antibodies, Monoclonal,
pubmed-meshheading:2422659-Antibodies, Viral,
pubmed-meshheading:2422659-Antibody Specificity,
pubmed-meshheading:2422659-B-Lymphocytes,
pubmed-meshheading:2422659-Cell Line,
pubmed-meshheading:2422659-Clone Cells,
pubmed-meshheading:2422659-Complement System Proteins,
pubmed-meshheading:2422659-Cytotoxicity, Immunologic,
pubmed-meshheading:2422659-Deltaretrovirus,
pubmed-meshheading:2422659-Epitopes,
pubmed-meshheading:2422659-Humans,
pubmed-meshheading:2422659-Leukemia,
pubmed-meshheading:2422659-Viral Envelope Proteins
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pubmed:year |
1986
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pubmed:articleTitle |
Human monoclonal antibody directed against an envelope glycoprotein of human T-cell leukemia virus type I.
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pubmed:publicationType |
Journal Article
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