Linked Life Data

2422074

Source:http://linkedlifedata.com/resource/pubmed/id/2422074

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-5-30
pubmed:abstractText
The synthesis of plasma proteins directed by mRNA from human brain tissues was studied by combining in vitro or in ovo translation of mRNAs with crossed immunoelectrophoresis of the mRNA-directed labeled polypeptides, followed by autoradiography of the washed plates. Poly(A)-containing mRNA was prepared from different developmental stages of fetal and postnatal human brain and also from primary glioblastomas and meningiomas. Several plasma protein-like polypeptides were identified in the autoradiographs by their migration coordinates in the two-dimensional gels, compared with immunoprecipitates formed by mature, unlabeled, stainable proteins. These included polypeptides migrating like Gc globulin, haptoglobin, fibrinogen, alpha-fetoprotein, transferrin, cholinesterase, and alpha 2-macroglobulin; other, yet unidentified plasma proteins, were also observed. In general, the synthesis of these plasma proteins appeared to be more pronounced in fetal and neoplastic brain tissues than in postnatal tissues. However, clear immunoprecipitates for some of these plasma proteins could also be detected in products directed by mRNA from particular regions of mature, normal brains, indicating that some synthesis of plasma proteins takes place in the human brain even as late as 40 years of age. mRNAs for several proteins were also identified in samples of neoplastic brain. mRNA for transferrin was identified in normal fetal and adult brain but not in either the glioblastomas or meningiomas studied. Microinjected Xenopus oocytes, in which post-translational processing occurs as well, were also used to translate fetal brain mRNA. Several plasma proteins could be detected in the translation products which were induced and stored in the oocytes. These included hemopexin, which could not be detected in the in vitro system. Others, such as cholinesterase, were found to be secreted by the oocytes. These findings indicate that different cell types in the human brain may produce and either store or secrete particular plasma proteins at defined stages in their development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:volume
115
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-104
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:2422074-Animals, pubmed-meshheading:2422074-Autoradiography, pubmed-meshheading:2422074-Blood Proteins, pubmed-meshheading:2422074-Brain, pubmed-meshheading:2422074-Brain Neoplasms, pubmed-meshheading:2422074-Cholinesterases, pubmed-meshheading:2422074-Fetus, pubmed-meshheading:2422074-Fibrinogen, pubmed-meshheading:2422074-Glioma, pubmed-meshheading:2422074-Haptoglobins, pubmed-meshheading:2422074-Humans, pubmed-meshheading:2422074-Immunoelectrophoresis, Two-Dimensional, pubmed-meshheading:2422074-Meningioma, pubmed-meshheading:2422074-Oocytes, pubmed-meshheading:2422074-Protein Biosynthesis, pubmed-meshheading:2422074-RNA, Messenger, pubmed-meshheading:2422074-Transferrin, pubmed-meshheading:2422074-Vitamin D-Binding Protein, pubmed-meshheading:2422074-Xenopus laevis, pubmed-meshheading:2422074-alpha 1-Antitrypsin, pubmed-meshheading:2422074-alpha-Fetoproteins, pubmed-meshheading:2422074-alpha-Macroglobulins
pubmed:year
1986
pubmed:articleTitle
Synthesis of plasma proteins in fetal, adult, and neoplastic human brain tissue.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't