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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1986-5-14
|
| pubmed:abstractText |
Serum from rabbits bearing tumors (TBR serum) induced by the Rous sarcoma virus (RSV) was originally developed to identify the RSV src gene protein-pp60v-src. It is also capable of directly or indirectly immunoprecipitating a number of proteins besides pp60v-src from lysates of RSV-transformed cells. This report describes a highly phosphorylated protein of approximately 120,000 Da (pp120) which is specifically immunoprecipitated from RSV-transformed mammalian cells by TBR sera and monospecific antibodies against pp60v-src. However, it was not immunoprecipitated by TBR serum from RSV-transformed chicken embryo fibroblasts. Phosphoamino acid analysis revealed that pp120 contains phosphoserine and phosphotyrosine in relative amounts similar to that found in pp60v-src. Various experimental results indicate that pp120 is not structurally related to RSV virion proteins or to the putative pp60v-src substrate-vinculin. Furthermore, proteolytic peptide mapping and immunoblotting experiments indicate that although pp120 is immunoprecipitated by various anti-pp60v-src antibodies, it does not appear to be related to the RSV transforming protein. These results suggest that pp120 is distinct from previously reported substrates of pp60v-src and may exist in association with the transforming protein in mammalian cell lysates.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Protein pp60(v-src),
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoserine,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Vinculin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0042-6822
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
151
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
86-99
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:2421483-Animals,
pubmed-meshheading:2421483-Arvicolinae,
pubmed-meshheading:2421483-Avian Sarcoma Viruses,
pubmed-meshheading:2421483-Cell Line,
pubmed-meshheading:2421483-Cell Transformation, Viral,
pubmed-meshheading:2421483-Chemical Precipitation,
pubmed-meshheading:2421483-Chick Embryo,
pubmed-meshheading:2421483-Epitopes,
pubmed-meshheading:2421483-Immune Sera,
pubmed-meshheading:2421483-Mice,
pubmed-meshheading:2421483-Molecular Weight,
pubmed-meshheading:2421483-Muscle Proteins,
pubmed-meshheading:2421483-Oncogene Protein pp60(v-src),
pubmed-meshheading:2421483-Phosphoproteins,
pubmed-meshheading:2421483-Phosphoserine,
pubmed-meshheading:2421483-Phosphotyrosine,
pubmed-meshheading:2421483-Retroviridae Proteins,
pubmed-meshheading:2421483-Tyrosine,
pubmed-meshheading:2421483-Vinculin
|
| pubmed:year |
1986
|
| pubmed:articleTitle |
Phosphotyrosine-containing 120,000-dalton protein coimmunoprecipitated with pp60v-src from Rous sarcoma virus-transformed mammalian cells.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|