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rdf:type |
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lifeskim:mentions |
umls-concept:C0007452,
umls-concept:C0016964,
umls-concept:C0020792,
umls-concept:C0205470,
umls-concept:C0220908,
umls-concept:C0439851,
umls-concept:C0702242,
umls-concept:C1515563,
umls-concept:C1517023,
umls-concept:C1522642,
umls-concept:C1552596,
umls-concept:C1947931
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pubmed:issue |
6
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pubmed:dateCreated |
1986-4-24
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pubmed:databankReference |
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pubmed:abstractText |
A 1.3-kilobase cDNA clone (7A) coding for bovine galactosyltransferase (glycoprotein 4-beta-galactosyltransferase, EC 2.4.1.38) was isolated from a lambda gt11 expression library by immunological screening with monospecific polyclonal antisera to the affinity-purified bovine enzyme. The nucleotide sequence of this clone predicts an open reading frame that starts at the 5' end of the insert and codes for a polypeptide of 334 amino acids with Mr 37,645. Based on a Mr of 57,000 for the membrane-bound enzyme this clone accounts for approximately 61% of the coding sequence. Portions of the predicted amino acid sequence matched the six tryptic peptides isolated from affinity-purified bovine galactosyltransferase. Clone 7A hybridizes to a 4.8-kilobase bovine mRNA and identifies multiple EcoRI restriction fragments in bovine, murine, and human DNA.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-2580642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-2995408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-3917437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-3934183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-402369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-4674085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-5132678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-5476326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-5719192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-6023212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-6121807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-6198242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-6356359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-6423427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-6800883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-6806257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-6815212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-6815262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2419911-6993404
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1573-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2419911-Amino Acid Sequence,
pubmed-meshheading:2419911-Animals,
pubmed-meshheading:2419911-Antibodies,
pubmed-meshheading:2419911-Bacteriophage lambda,
pubmed-meshheading:2419911-Base Sequence,
pubmed-meshheading:2419911-Cattle,
pubmed-meshheading:2419911-Cell Line,
pubmed-meshheading:2419911-Chromatography, Affinity,
pubmed-meshheading:2419911-DNA,
pubmed-meshheading:2419911-DNA, Recombinant,
pubmed-meshheading:2419911-Galactosyltransferases,
pubmed-meshheading:2419911-Genetic Markers,
pubmed-meshheading:2419911-Humans,
pubmed-meshheading:2419911-Kidney,
pubmed-meshheading:2419911-Membrane Proteins,
pubmed-meshheading:2419911-Mice,
pubmed-meshheading:2419911-RNA,
pubmed-meshheading:2419911-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2419911-beta-N-Acetylglucosaminylglycopeptide...
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pubmed:year |
1986
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pubmed:articleTitle |
Bovine galactosyltransferase: identification of a clone by direct immunological screening of a cDNA expression library.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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