Linked Life Data

2419522

Source:http://linkedlifedata.com/resource/pubmed/id/2419522

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1986-4-17
pubmed:abstractText
Cytochemically stained acetylcholinesterase (AChE) activity in endplate regions of adult rat gracilis muscles was studied after in situ treatment with AChE inhibitors which differ in lipid solubility and hence in their ability to penetrate cell membranes. Control preparations showed intense AChE staining over junctional infoldings and within myofibres, but little enzymatic reaction product in nerve terminals and Schwann cells. Echothiophate (poorly lipid soluble) drastically reduced only extracellular AChE activity, whereas sequential treatment with BW284C51 (poorly lipid soluble) and diisopropylfluorophosphate (lipid soluble) primarily eliminated intracellular AChE. Extracellular AChE activity (associated with the synaptic basal lamina) was predominantly composed of asymmetric enzymatic forms. Intracellular AChE (associated with the sarcoplasmic reticulum of the myofibres) primarily contained globular forms and a small proportion of asymmetric forms. Little or no external AChE activity was detected in non-endplate muscle regions and the internal enzyme was confined to a restricted subcellular region close to the point of innervation. These results establish the validity of using the above in situ pharmacological treatments to demonstrate intracellular and extracellular pools of AChE in adult skeletal muscles. In addition, they are consistent with the idea that motor neurons play an essential role in the mechanisms which determine the subcellular distribution of AChE.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0300-4864
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
795-808
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Cellular localization of cytochemically stained acetylcholinesterase activity in adult rat skeletal muscle.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't