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rdf:type |
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|
lifeskim:mentions |
|
|
pubmed:issue |
2
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pubmed:dateCreated |
1986-4-4
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pubmed:abstractText |
The nicotinic acetylcholine receptor from Torpedo marmorata was digested using papain and the reaction products separated by SDS gel electrophoresis and characterised by immunoblotting using labelled alpha-bungarotoxin, polyclonal antibodies to synthetic peptides and monoclonal antibodies to the main immunogenic region (MIR). Using this approach, it was possible to show that the MIR is located N-terminal to all or part of peptide 151-169 (peptide P1) of the alpha-chain and that papain cleaves the alpha-chain between Asn 141 and peptide P1.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-5793
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
17
|
|
pubmed:volume |
196
|
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
237-41
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2419157-Animals,
pubmed-meshheading:2419157-Antibodies, Monoclonal,
pubmed-meshheading:2419157-Binding Sites,
pubmed-meshheading:2419157-Bungarotoxins,
pubmed-meshheading:2419157-Epitopes,
pubmed-meshheading:2419157-Immunochemistry,
pubmed-meshheading:2419157-Papain,
pubmed-meshheading:2419157-Peptides,
pubmed-meshheading:2419157-Protein Conformation,
pubmed-meshheading:2419157-Receptors, Nicotinic,
pubmed-meshheading:2419157-Torpedo
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pubmed:year |
1986
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pubmed:articleTitle |
Localisation of the main immunogenic region of the nicotinic acetylcholine receptor.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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