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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1986-3-21
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pubmed:abstractText |
The effect of dihydropyridine agonists and antagonists on neuronal voltage sensitive calcium channels was investigated. The resting intracellular calcium concentration of synaptosomes prepared from whole brain was 110 +/- 9 nM, as assayed by the indicator quin 2. Depolarisation of the synaptosomes with K+ produced an immediate increase in [Ca2+]i. The calcium agonist Bay K 8644 and antagonist nifedipine did not affect [Ca2+]i under resting or depolarising conditions. In addition, K+ stimulated 45Ca2+ uptake into synaptosomes prepared from the hippocampus was insensitive to Bay K 8644 and PY 108-068 in normal or Na+ free conditions. In neuronally derived NG108-15 cells the enantiomers of the dihydropyridine derivative 202-791 showed opposite effects in modulating K+ stimulated 45Ca2+ uptake. (-)-R-202-791 inhibited K+ induced 45Ca2+ uptake with an IC50 of 100 nM and (+)-S-202-791 enhanced K+ stimulated uptake with an EC50 of 80 nM. These results suggest that synaptosomal voltage sensitive calcium channels either are of a different type to those found in peripheral tissues and cells of neural origin or that expression of functional effects of dihydropyridines requires different experimental conditions to those used here.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,4-dihydropyridine,
http://linkedlifedata.com/resource/pubmed/chemical/3-Pyridinecarboxylic acid...,
http://linkedlifedata.com/resource/pubmed/chemical/Aminoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Dihydropyridines,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Nifedipine,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/Quin2,
http://linkedlifedata.com/resource/pubmed/chemical/Verapamil,
http://linkedlifedata.com/resource/pubmed/chemical/darodipine
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
134
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1038-47
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2418832-3-Pyridinecarboxylic acid...,
pubmed-meshheading:2418832-Aminoquinolines,
pubmed-meshheading:2418832-Animals,
pubmed-meshheading:2418832-Brain Chemistry,
pubmed-meshheading:2418832-Calcium,
pubmed-meshheading:2418832-Calcium Channel Blockers,
pubmed-meshheading:2418832-Cell Line,
pubmed-meshheading:2418832-Dihydropyridines,
pubmed-meshheading:2418832-Electric Stimulation,
pubmed-meshheading:2418832-Glioma,
pubmed-meshheading:2418832-Ion Channels,
pubmed-meshheading:2418832-Neuroblastoma,
pubmed-meshheading:2418832-Neurons,
pubmed-meshheading:2418832-Nifedipine,
pubmed-meshheading:2418832-Potassium,
pubmed-meshheading:2418832-Pyridines,
pubmed-meshheading:2418832-Rats,
pubmed-meshheading:2418832-Synaptosomes,
pubmed-meshheading:2418832-Verapamil
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pubmed:year |
1986
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pubmed:articleTitle |
The effect of dihydropyridine calcium agonists and antagonists on neuronal voltage sensitive calcium channels.
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pubmed:publicationType |
Journal Article,
In Vitro
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