Linked Life Data

2418827

Source:http://linkedlifedata.com/resource/pubmed/id/2418827

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-3-14
pubmed:abstractText
Synthesis and secretion of alpha 2-macroglobulin were studied with the human lung fibroblast cell line GM 1379. After incubation with [3H]leucine the cells secreted radioactively labeled alpha 2-macroglobulin consisting of subunits with a molecular weight of 180,000. When the cells were treated with tunicamycin the unglycosylated alpha 2-macroglobulin subunit exhibited a molecular weight of 160,000. Poly(A) +RNA was isolated from the cultured cells and translated in a rabbit reticulocyte lysate. From the translation products an alpha 2-macroglobulin species with a molecular weight of 160,000 was immunoprecipitated. The addition of pancreatic microsoms to the translation mixture resulted in the synthesis of an alpha 2-macroglobulin subunit which had molecular weights of 180,000. Thus, a size of approximately 160,000 for the protein moiety and 20,000 for the carbohydrate portion can be estimated for a subunit of alpha 2-macroglobulin from human lung fibroblasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
134
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-63
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Synthesis and secretion of alpha 2-macroglobulin by human lung fibroblasts.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't