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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1986-3-14
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pubmed:abstractText |
Polyclonal reagents have been used to define HLA class II molecules in conventional serologic and cellular typing. We generated human alloreactive T-cell clones to analyze the functional fine specificities of HLA class II molecules that might be important for the phenomenon of HLA and disease association. We chose to examine HLA-Dw14, an HLA-D specificity that has been associated with juvenile rheumatoid arthritis. In this paper we have presented data that suggest that conventional cellular typing does not reflect the distribution of T-cell epitopes on major histocompatibility complex class II molecules. We describe three alloreactive T-cell clones that have defined three separate Dw14-associated T-cell epitopes. Two of these epitopes were on a DR-region molecule; the third was located on a DQ-region product. In a panel of unrelated DR4-positive donors, these three DW14-associated determinants were present in a high frequency but were not linked to each other. Within the tested panel of DR4-positive cells, all possible combinatorial arrangements of these three allodeterminants were seen. The concurrent expression of any two of the three allodeterminants was equivalent to a positive typing response for Dw14. Our finding that HLA-Dw14 is not characterized by a unique allodeterminant but by the combinatorial recognition of independently distributed T-cell interaction sites suggests that analysis of HLA and disease association may be more clearly demonstrated through the use of human T-cell clones.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-12735313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-12735314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-140623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-2578218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-4130064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-56405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6181033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6181034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6189938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6189945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6192342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6332101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6339368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6417660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6420500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6610692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6787587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6966655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-6979750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-76289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2418442-93286
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
762-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2418442-Antibodies, Monoclonal,
pubmed-meshheading:2418442-Cell Line,
pubmed-meshheading:2418442-Clone Cells,
pubmed-meshheading:2418442-Epitopes,
pubmed-meshheading:2418442-HLA-DQ Antigens,
pubmed-meshheading:2418442-HLA-DR4 Antigen,
pubmed-meshheading:2418442-Haploidy,
pubmed-meshheading:2418442-Histocompatibility Antigens Class II,
pubmed-meshheading:2418442-Humans,
pubmed-meshheading:2418442-T-Lymphocytes
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pubmed:year |
1986
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pubmed:articleTitle |
Human T-cell clones used to define functional epitopes on HLA class II molecules.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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