Linked Life Data

2418117

Source:http://linkedlifedata.com/resource/pubmed/id/2418117

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-3-7
pubmed:abstractText
It has been demonstrated previously that draining lymph nodes (DLN) from tumor-immunized mice contain a population of lymphoid cells that are capable of differentiating into functional antitumor cytotoxic T lymphocytes (CTL) during in vitro culture. In the present studies, it was observed that DLN cells from either C57BL/10 (B10) or C3H mice that had been footpad-immunized with syngeneic tumor cells differentiated into CTL during a 4-day in vitro culture in the absence of added antigen. The specificity patterns of the CTL thus generated, however, were quite different in the two strains. DLN from B10 mice immunized with ultraviolet light-induced fibrosarcoma cells of B10 origin differentiated into CTL which were only capable of lysing target cells from the tumor used for immunization. Thus, the antitumor CTL which differentiate from B10 DLN appeared to be specific for the tumor-specific antigen (TSA) expressed by these tumor cells. In contrast, DLN from C3H mice immunized with a syngeneic ultraviolet light-induced fibrosarcoma differentiated into CTL which effectively lysed not only target cells from the immunizing tumor, but several other fibrosarcomas of both B10 and C3H origin, and which did not lyse normal nontumor targets. These C3H effectors thus appeared to be specific for a tumor-associated antigen (TAA) which is widely shared by a number of tumors. Cold target-blocking studies demonstrated that the CTL generated by C3H DLN cells contained a subpopulation of TSA-specific cells in addition to cross-reactive TAA-specific effectors. (B6 X C3H)F1 (B6C3F1) mice generated cross-reactive TAA-specific CTL in response to in vivo challenge with either B10 or C3H tumors, indicating that the ability to generate a TAA-specific CTL response behaves as a dominant trait of the responding mouse strain and not as a function of the tumor used for immunization. TSA-specific CTL and cross-reactive TAA-specific CTL were distinguishable on the basis of their cell surface phenotypes, because the TSA-specific CTL generated by B10 DLN cells were Thy-1.2+ Lyt-2.2+, whereas TAA-specific B6C3F1 CTL were Thy-1.2+ Lyt-2.2-; alloantigen-specific CTL generated from the same B6C3F1 lymph nodes were Thy-1.2+ Lyt-2.2+.(ABSTRACT TRUNCATED AT 400 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
136
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1521-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2418117-Animals, pubmed-meshheading:2418117-Antigens, Ly, pubmed-meshheading:2418117-Antigens, Neoplasm, pubmed-meshheading:2418117-Antigens, Surface, pubmed-meshheading:2418117-Epitopes, pubmed-meshheading:2418117-Fibrosarcoma, pubmed-meshheading:2418117-Histocompatibility Antigens, pubmed-meshheading:2418117-Immunity, Cellular, pubmed-meshheading:2418117-Lymph Nodes, pubmed-meshheading:2418117-Lymphocyte Activation, pubmed-meshheading:2418117-Lymphoma, pubmed-meshheading:2418117-Mice, pubmed-meshheading:2418117-Mice, Inbred BALB C, pubmed-meshheading:2418117-Mice, Inbred C3H, pubmed-meshheading:2418117-Mice, Inbred C57BL, pubmed-meshheading:2418117-Neoplasm Transplantation, pubmed-meshheading:2418117-Phenotype, pubmed-meshheading:2418117-Species Specificity, pubmed-meshheading:2418117-T-Lymphocytes, Cytotoxic, pubmed-meshheading:2418117-Transplantation, Isogeneic
pubmed:year
1986
pubmed:articleTitle
Cell-mediated immune responses to syngeneic tumors. I. Identification of two distinct CTL effector pathways which differ in antigen specificity, genetic regulation, and cell surface phenotype.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.