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rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
21
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pubmed:dateCreated |
1985-12-23
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pubmed:abstractText |
Gliclazide(hypoglycemic drug having sulfonylurea structure)-degrading activity was found in fraction M(macroglobulin, Fr. M) obtained from pooled human serum by gel filtration using a Sephadex G-150 column. The main degrading activity was in the fraction eluted from the Fr. M-subjected DEAE-cellulose column with 0.4 M phosphate buffer (pH 5.2), and the gliclazide-degrading protein localized around alpha 2 to beta-globulin on an electrophoretic pattern using a cellulose acetate membrane. The degrading activity was enhanced about two-fold by lyophilizing Fr. M solution containing a higher sodium phosphate (Na2HPO4-NaH2PO4), over 0.27 M. This indicates that the appearance and enhancement of the degrading activity required the combination of the lyophilization of the sample solution and a certain initial concentration of sodium phosphate prior to lyophilization.
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pubmed:language |
eng
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pubmed:journal |
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|
pubmed:citationSubset |
IM
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pubmed:chemical |
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|
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0024-3205
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2015-9
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2415795-Alpha-Globulins,
pubmed-meshheading:2415795-Beta-Globulins,
pubmed-meshheading:2415795-Chromatography, DEAE-Cellulose,
pubmed-meshheading:2415795-Chromatography, Gel,
pubmed-meshheading:2415795-Electrophoresis, Cellulose Acetate,
pubmed-meshheading:2415795-Freeze Drying,
pubmed-meshheading:2415795-Gliclazide,
pubmed-meshheading:2415795-Humans,
pubmed-meshheading:2415795-Phosphates,
pubmed-meshheading:2415795-Protein Conformation,
pubmed-meshheading:2415795-Sulfonylurea Compounds,
pubmed-meshheading:2415795-Ultrafiltration
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pubmed:year |
1985
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|
pubmed:articleTitle |
Influence of blood proteins on biomedical analysis. VIII. Attempts at purification of gliclazide-degrading factor in human serum.
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pubmed:publicationType |
Journal Article
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