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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
|
pubmed:dateCreated |
1985-11-21
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pubmed:abstractText |
A GAT-specific "second order" suppressor T cell factor (TsF2) from the hybridoma 762 B3.7 has been purified and biochemically characterized. The protein has a m.w. of approximately 66,000, an isoelectric point of 6.8 to 6.9, and elutes from a reversed phase HPLC column in two peaks, one in 55% acetonitrile, the other in 70% propanol. Amino acid analysis of both forms gave similar molar ratios, suggesting that the two forms are closely related and may differ mainly in the degree of posttranslational modification. SDS-PAGE electrophoresis under reducing conditions gave two chains of the apparent m.w. of 42,000 and 35,000.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
135
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3232-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2413116-Animals,
pubmed-meshheading:2413116-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2413116-Epitopes,
pubmed-meshheading:2413116-Hybridomas,
pubmed-meshheading:2413116-Isoelectric Focusing,
pubmed-meshheading:2413116-Mice,
pubmed-meshheading:2413116-Molecular Weight,
pubmed-meshheading:2413116-Peptides,
pubmed-meshheading:2413116-Precipitin Tests,
pubmed-meshheading:2413116-Suppressor Factors, Immunologic,
pubmed-meshheading:2413116-T-Lymphocytes
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pubmed:year |
1985
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pubmed:articleTitle |
Purification and partial characterization of a monoclonal "second order" suppressor factor specific for L-glutamic acid60-L-alanine30-L-tyrosine10.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|