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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1985-10-25
|
| pubmed:abstractText |
A pBR322-derived expression vector, plasmid pKD1, was constructed containing the strong leftward promoter (pL) of bacteriophage lambda, the ribosome-binding site (RBS) of the cII gene of lambda, and a unique downstream NdeI restriction site for construction of an ATG initiation codon. The section of the pol gene of Moloney murine leukemia virus (M-MLV) that codes for reverse transcriptase (RT) was cloned into the NdeI site of this vector generating the plasmid pRT103. Upon thermal induction, enzymatically active RT was expressed in Escherichia coli [pRT103]. The identity of this activity was confirmed by its template specificity and its sensitivity to inhibition by immunoglobulin G (IgG) prepared against authentic murine RT. RT represented 20% of the newly synthesized protein in these cells 20 min after induction.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0378-1119
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
35
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
249-58
|
| pubmed:dateRevised |
2001-11-2
|
| pubmed:meshHeading |
pubmed-meshheading:2412939-Bacteriophage lambda,
pubmed-meshheading:2412939-Cloning, Molecular,
pubmed-meshheading:2412939-DNA, Recombinant,
pubmed-meshheading:2412939-Escherichia coli,
pubmed-meshheading:2412939-Gene Expression Regulation,
pubmed-meshheading:2412939-Molecular Weight,
pubmed-meshheading:2412939-Moloney murine leukemia virus,
pubmed-meshheading:2412939-Protein Conformation,
pubmed-meshheading:2412939-RNA-Directed DNA Polymerase
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase in Escherichia coli.
|
| pubmed:publicationType |
Journal Article
|