Linked Life Data

2412676

Source:http://linkedlifedata.com/resource/pubmed/id/2412676

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1985-11-21
pubmed:abstractText
A method for dissociating and culturing myoblast cells from normal (golden Syrian) and myopathic (CHF-146) hind leg muscles of 8- to 10-day-old hamsters has been described. We have compared the fusion ability of the myoblasts from normal tissue with that of myopathic myoblasts and, further, we have studied this process with respect to gelatin-coated and uncoated plastic culture dishes. Our data show that myoblasts from normal tissue grows well on both surfaces, although fusion is enhanced when these cells are grown on a gelatin-coated surface. Myoblasts from dystrophic tissue, however, appear to have an impaired ability to adhere to an uncoated surface, although fusion is enhanced when these myoblasts are grown on coated plates. The fusion percentage of the dystrophic cells is consistently lower than that of myoblasts from normal tissue. At low concentrations of 5-azacytidine tested, there seems to be no enhancement of fusion capability in either the normal or dystrophic lines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0714-7511
pubmed:author
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
730-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Characteristics of myoblasts isolated from golden Syrian and dystrophic (strain CHF-146) hamsters.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't