Linked Life Data

2412365

Source:http://linkedlifedata.com/resource/pubmed/id/2412365

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-10-2
pubmed:abstractText
UV-irradiated Azotobacter vinelandii OP cells were found to be sensitive to dark repair-inhibitors, like caffeine and acriflavine. Sensitivity to both the inhibitors started to decrease at a fast rate immediately following UV-irradiation, when incubated in Burk's broth in dark. Total insensitivity to caffeine was attained at a time that was approximately double the time of the corresponding ribonucleic acid synthesis-inhibition and less than one generation time of the unirradiated cells. Nearly at the same time the rate of loss of sensitivity to acriflavine became much slower and total insensitivity to acriflavine occurred after relatively prolonged incubation. The protein synthesis inhibitor, streptomycin, reduced UV-survival, but after a time lag. The organism did not show liquid holding recovery and its UV-survival was not affected by components of complex medium. It is proposed that A. vinelandii OP possesses at least two dark-repair pathways: pre replication (acts immediately after UV exposure and is faster) and post replication (delayed and slower) repair. The first one is inhibited by caffeine and acriflavine and the second by acriflavine and streptomycin. The latter therefore requires de novo protein synthesis after UV-irradiation and may be inducible.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0232-4393
pubmed:author
pubmed:issnType
Print
pubmed:volume
140
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
247-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
UV-repair and mutagenesis in Azotobacter vinelandii. I. Repair of UV-induced damages.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't