rdf:type |
|
lifeskim:mentions |
umls-concept:C0001455,
umls-concept:C0007600,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0040132,
umls-concept:C0040160,
umls-concept:C0178702,
umls-concept:C0441712,
umls-concept:C0871261,
umls-concept:C1522642,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1948023,
umls-concept:C2603343,
umls-concept:C2911692
|
pubmed:issue |
1
|
pubmed:dateCreated |
1985-10-18
|
pubmed:abstractText |
We examined aspects of the mechanism of desensitization of adenylate cyclase activation by TSH in a cloned line of rat thyroid cells (FRTL). Increasing FRTL intracellular cAMP concentrations by preincubation for 6 h in either 1 mM dBcAMP or 100 microM forskolin did not induce TSH desensitization. Forskolin stimulation was unimpaired in TSH-desensitized cells, indicating 'uncoupling' of the adenylate cyclase catalytic unit from the TSH receptor. Stimulation by the Ni inhibitory pathway of the adenylate cyclase by epinephrine (10(-6) M-10(-4) M in the presence of 10(-4) M propranolol) was unaltered in cells previously desensitized to TSH. That is, Ni-mediated inhibition of adenylate cyclase was additive to TSH desensitization. Pre-exposure of FRTL cells for 18 h to 50 ng/ml pertussis toxin did not prevent the induction of TSH desensitization. TSH desensitization was prevented by cycloheximide or actinomycin D added during the last 3-4 h of a 6 h period of TSH stimulation. The rates of turnover of the putative desensitization protein and its mRNA therefore appear to be similar.
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pubmed:grant |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0303-7207
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
42
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
21-7
|
pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2411613-Adenylate Cyclase,
pubmed-meshheading:2411613-Animals,
pubmed-meshheading:2411613-Cells, Cultured,
pubmed-meshheading:2411613-Cyclic AMP,
pubmed-meshheading:2411613-Enzyme Activation,
pubmed-meshheading:2411613-GTP-Binding Proteins,
pubmed-meshheading:2411613-Protein Biosynthesis,
pubmed-meshheading:2411613-RNA,
pubmed-meshheading:2411613-Rats,
pubmed-meshheading:2411613-Receptors, Cell Surface,
pubmed-meshheading:2411613-Receptors, Thyrotropin,
pubmed-meshheading:2411613-Thyroid Gland,
pubmed-meshheading:2411613-Thyrotropin
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pubmed:year |
1985
|
pubmed:articleTitle |
Studies on the mechanism of desensitization of the cyclic AMP response to TSH stimulation in a cloned rat thyroid cell line.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|