Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1985-10-7
|
pubmed:abstractText |
Antibody 3-40 defines a 35- to 40-kd surface antigen present on T lymphoblastic leukemia (T-ALL) cells that is absent on normal hematopoietic cells (Naito et al, Blood 62:852, 1983). Using immunoblot analysis of cytoskeletal proteins and indirect immunofluorescence of cell lines treated with various cytoskeletal inhibitors, in this report we have demonstrated that antibody 3-40 also identifies vimentin intermediate filaments (IMF) within the HSB-2 (T-ALL) cell line as well as in normal thymocytes, peripheral blood mononuclear cells, and human and rodent fibroblast cell lines. Cross-reactivity with several keratin subclasses was demonstrated in both human and rodent epithelial cell lines, human thymus, and skin. In addition, we have shown that antibody 3-40 defined a 39-kd intracellular IMF-associated protein in HSB-2 cells, epithelial and fibroblast cell lines. This IMF-associated protein may be selectively expressed on the surface of human T cells during malignant transformation.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0006-4971
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
66
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
642-8
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:2411311-Animals,
pubmed-meshheading:2411311-Antibodies, Neoplasm,
pubmed-meshheading:2411311-Antigen-Antibody Reactions,
pubmed-meshheading:2411311-Antigens, Neoplasm,
pubmed-meshheading:2411311-Cell Line,
pubmed-meshheading:2411311-Cytoskeleton,
pubmed-meshheading:2411311-Epithelium,
pubmed-meshheading:2411311-Fibroblasts,
pubmed-meshheading:2411311-Humans,
pubmed-meshheading:2411311-Keratins,
pubmed-meshheading:2411311-Rats,
pubmed-meshheading:2411311-Subcellular Fractions,
pubmed-meshheading:2411311-T-Lymphocytes,
pubmed-meshheading:2411311-Vimentin
|
pubmed:year |
1985
|
pubmed:articleTitle |
Antibody against T lymphoblastic leukemia-associated antigen (3-40) identifies vimentin and keratin intermediate filaments in normal cells.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|