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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1985-9-4
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pubmed:abstractText |
Circular dichroism (CD) spectra in the vacuum UV region for different conformations of poly d(G-C) X poly d(G-C) and poly r(G-C) X poly r(G-C) are very characteristic. The CD of the RNA in the A-form (6 M NaClO4 and 22 degrees C) is very similar to that of the DNA in 80% alcohol where it is believed to be in the A-form. With the exception of the longest wavelength transition, the CD of the RNA in 6 M NaClO4 at 46 degrees C is similar to the CD of the DNA under conditions where it is believed to be in the Z-form (2 M NaClO4). This substantiates that poly r(G-C) X poly r(G-C) assumes a left-handed Z-conformation in 6 M NaClO4 above 35 degrees C. CD spectra for the left-handed Z-forms of both the RNA and DNA are characterized by an intense negative peak at 190-195 nm, a crossover at about 184 nm, and an intense positive peak below 180 nm. The right-handed A- and B-forms of RNA and DNA all have an intense positive peak in their CD spectra near 186 nm. The large difference in CD in the range 185-195 nm for right- and left-handed conformations of nucleic acids can be used to identify the sense of helix winding.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-1256581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-14220659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-288040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-424316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-435605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-4593439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-4600810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-4687151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-5045303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-5094948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-5492018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-5915178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-6095898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-6693410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-6946428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-6951185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-7402336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-7444458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2410859-7463477
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4983-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1985
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pubmed:articleTitle |
Evidence for Z-form RNA by vacuum UV circular dichroism.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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