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rdf:type |
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lifeskim:mentions |
umls-concept:C0012939,
umls-concept:C0033731,
umls-concept:C0035380,
umls-concept:C0035668,
umls-concept:C0204727,
umls-concept:C0205177,
umls-concept:C0205409,
umls-concept:C0220781,
umls-concept:C0439750,
umls-concept:C0446302,
umls-concept:C1264633,
umls-concept:C1704973,
umls-concept:C1705542,
umls-concept:C1883254
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pubmed:issue |
12
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pubmed:dateCreated |
1985-8-16
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pubmed:abstractText |
Sub-cellular fractions, isolated from cauliflower mosaic virus (CaMV)-infected turnip protoplasts, are capable of synthesising CaMV DNA in vitro on an endogenous template and of reverse transcribing oligo dT-primed cowpea mosaic virus RNA. The activity was not detected in mock-inoculated protoplasts. In vitro-labelled DNA hybridized to single-stranded M13 clones complementary to the putative origins of (-) and (+) strand CaMV DNA synthesis and to restriction endonuclease fragments encompassing more than 90% of the CaMV genome. The synthesis of (-) and (+) strand DNA appeared asymmetric. The template(s) for in vitro CaMV DNA synthesis are in a partially nuclease-resistant form. Fractions capable of in vitro CaMV DNA synthesis contained CaMV RNA both heterogeneous and as discrete species; they also contained a range of different sizes of CaMV DNA. Several lines of evidence indicate that this range of in vitro-labelled CaMV DNA, extending from 0.6kb to 8.0kb in length, represents elongating (-) strand DNA. These are discussed in relation to their role as possible replicative intermediates.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-11894938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-16453487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-16453500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-4706708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-503861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-559371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-5777571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6154876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6180831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6187624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6191868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6195530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6199741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6260957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6269062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6276092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6287459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6298717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6300783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6495660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6495661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6514573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6628362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-664242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-664243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6662362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-6863467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-7139714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-7152260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-7350544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-7407912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409536-881736
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4557-76
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:2409536-Cloning, Molecular,
pubmed-meshheading:2409536-DNA,
pubmed-meshheading:2409536-DNA, Viral,
pubmed-meshheading:2409536-DNA Replication,
pubmed-meshheading:2409536-DNA Restriction Enzymes,
pubmed-meshheading:2409536-Molecular Weight,
pubmed-meshheading:2409536-Mosaic Viruses,
pubmed-meshheading:2409536-Nucleic Acid Hybridization,
pubmed-meshheading:2409536-Plants,
pubmed-meshheading:2409536-Protoplasts,
pubmed-meshheading:2409536-RNA-Directed DNA Polymerase,
pubmed-meshheading:2409536-Species Specificity,
pubmed-meshheading:2409536-Templates, Genetic
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pubmed:year |
1985
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pubmed:articleTitle |
Isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of (+) and (-) strand viral DNA and reverse transcription of primed RNA templates.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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