Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1985-8-19
|
| pubmed:abstractText |
Long-term culture of a nonadherent pre-B cell line was established from BALB/c bone marrow by the method of Whitlock and Witte. Adherent filler cells, also derived from bone marrow, were required for the growth of such pre-B cells. The surface phenotypes of the nonadherent cells determined by FACS analysis were negative for mu-chain, kappa-chain, and Ia, but 50% of nonadherent cells expressed a marker recognized by the monoclonal 14.8. Analysis of cytoplasmic immunofluorescent staining revealed the presence of cytoplasmic mu-chain but absence of kappa-chain. These pre-B cells did not respond in vitro to such mitogens as LPS or DxSO4 and were not stimulated by allospecific helper T cells. However, when such BALB/c derived pre-B cells were transferred to irradiated BDF1 mice, recovered spleen cells expressed mu-chain as well as the marker recognized by 14.8. Such in vivo passaged pre-B cells were able to respond to LPS in vitro. Moreover, the response observed was diminished markedly by anti-H-2d antiserum and complement (C) but not anti-H-2b and C, indicating that the responder cells were of donor rather than host origin. Because the reconstitution of irradiated mice was limited to cells of the B lymphocyte lineage, it would appear that cells recovered from in vivo passage are the progeny of in vitro cultured pre-B cells and not that of in vitro contaminating stem cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
135
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
965-72
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2409163-Animals,
pubmed-meshheading:2409163-B-Lymphocytes,
pubmed-meshheading:2409163-Bone Marrow Cells,
pubmed-meshheading:2409163-Bone Marrow Transplantation,
pubmed-meshheading:2409163-Cell Differentiation,
pubmed-meshheading:2409163-Cells, Cultured,
pubmed-meshheading:2409163-Dextran Sulfate,
pubmed-meshheading:2409163-Dextrans,
pubmed-meshheading:2409163-Female,
pubmed-meshheading:2409163-Immunization, Passive,
pubmed-meshheading:2409163-Lipopolysaccharides,
pubmed-meshheading:2409163-Lymphocyte Activation,
pubmed-meshheading:2409163-Mice,
pubmed-meshheading:2409163-Mice, Inbred BALB C,
pubmed-meshheading:2409163-Mice, Inbred C3H,
pubmed-meshheading:2409163-Mice, Inbred C57BL,
pubmed-meshheading:2409163-Mice, Inbred DBA,
pubmed-meshheading:2409163-Phenotype,
pubmed-meshheading:2409163-Rats,
pubmed-meshheading:2409163-Stem Cells,
pubmed-meshheading:2409163-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:2409163-Time Factors
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
In vivo maturation of pre-B cells derived from long-term cultured bone marrow.
|
| pubmed:publicationType |
Journal Article
|