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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-8-22
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pubmed:abstractText |
F-like conjugative pili are expressed by plasmids with closely related transfer systems. They are tubular filaments that are composed of repeating pilin subunits arranged in a helical array. Both F and ColB2 pilin have nearly identical protein sequences, and both contain an acetylated amino-terminal alanine residue. However, they differ by a few amino acid residues at their amino termini. Rabbit antisera raised against purified F and ColB2 pili are immunologically cross-reactive by only 25%, as measured by a competition enzyme-linked immunosorbent assay (ELISA). A tryptic peptide corresponding to the first 15 amino acid residues of ColB2 pilin was isolated and found to remove nearly 80% of ColB2 pilus-directed rabbit antibodies. The corresponding tryptic peptide from F pilin, which reacted with anti-F pilus antibodies to remove 80%, was less than 20% reactive with anti-ColB2 pilus antiserum. Cleavage of these peptides with cyanogen bromide (at a methionine residue approximately in the middle of the peptide) did not affect the antigenicity of these peptides. Synthetic N alpha-acetylated peptides corresponding to the first eight amino acids of F pilin (Ac-Ala-Gly-Ser-Ser-Gly-Gln-Asp-Leu-COOH) and the first six amino acids of ColB2 pilin (Ac-Ala-Gln-Gly-Gln-Asp-Leu-COOH) were prepared and tested by competition ELISA with homologous and heterologous anti-pilus antisera. The F peptide F(1-8) inhibited the interaction of F pili and anti-F pilus antiserum to 80%, while the ColB2 peptide ColB2(1-6) inhibited anti-ColB2 pilus antiserum reacting with ColB2 pili by greater than 60%. The two peptides F(1-8) and ColB2(1-6) were inactive by competition ELISAs with heterologous antisera. These results suggest that the major antigenic determinant of both F and ColB2 pili is at the amino terminus of the pilin subunit and that 80% of antibodies raised against these pili are specific for this region of the pilin molecule.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-1003480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-2578457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-339109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-357679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-381672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-4106220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-4114944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-4345849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-4907586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-4925086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-5338586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6090426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6090427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6110653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6111288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6120442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6130062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6145171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6185467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6194112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6201705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6219273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-6379416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2409073-7026236
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
331-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2409073-Amino Acid Sequence,
pubmed-meshheading:2409073-Antigens, Bacterial,
pubmed-meshheading:2409073-Bacterial Outer Membrane Proteins,
pubmed-meshheading:2409073-Bacteriocin Plasmids,
pubmed-meshheading:2409073-Cross Reactions,
pubmed-meshheading:2409073-Epitopes,
pubmed-meshheading:2409073-Escherichia coli,
pubmed-meshheading:2409073-Escherichia coli Proteins,
pubmed-meshheading:2409073-F Factor,
pubmed-meshheading:2409073-Fimbriae, Bacterial,
pubmed-meshheading:2409073-Fimbriae Proteins,
pubmed-meshheading:2409073-Oligopeptides,
pubmed-meshheading:2409073-Species Specificity
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pubmed:year |
1985
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pubmed:articleTitle |
Major antigenic determinants of F and ColB2 pili.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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