Linked Life Data

2407782

Source:http://linkedlifedata.com/resource/pubmed/id/2407782

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1990-4-9
pubmed:abstractText
Two morphologically distinct primary cultures of murine thymic stroma were established and found to be of epithelial (MTEC) and mesenchymal (MTMC) origin. These cultures were generated by selective conditions of tissue disruption and were maintained on extracellular matrix in defined medium. Culture supernatants (CS) from these cultures (EC-CS and MC-CS respectively), were tested for cytokine production and for effects on thymocyte maturation. Both supernatants displayed the activities of IL-3 and of granulocyte/macrophage-CSF and not of IL-1, -2, -4, or IFN. In addition they were found to be mitogenic to murine thymocytes in a "spontaneous" [3H]TdR incorporation assay. The two supernatants differed, however, in their effect on Con A stimulation. EC-CS had a strong enhancing effect, both when used for preincubation (18 h) before Con A stimulation or when present simultaneously with it. MC-CS had a small inconsistent effect under these conditions. Also EC-CS enhanced IL-2 and IL-3 production by thymocytes. The responsive thymocyte subpopulation was the one that does not bind peanut agglutinin. CS of an established thymic epithelial cell line displayed only part of these activities at a considerably lower level. CS from primary kidney cell culture was completely devoid of activity. The results suggest that primary thymic stromal cell cultures, cultivated under the defined conditions described here, may better preserve physiologic secretory activities, and probably also other cell functions, compared with established cell lines. Furthermore, the results are compatible with the hypothesis that the soluble factors, secreted by thymic stromal cells, are active on either very early or late stages of thymic differentiation, whereas the main intrathymic stages of differentiation are conceivable dependent primarily on direct contact with stromal cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
144
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1563-70
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed-meshheading:2407782-Animals, pubmed-meshheading:2407782-Biological Factors, pubmed-meshheading:2407782-Cell Division, pubmed-meshheading:2407782-Cell Separation, pubmed-meshheading:2407782-Cells, Cultured, pubmed-meshheading:2407782-Colony-Stimulating Factors, pubmed-meshheading:2407782-Concanavalin A, pubmed-meshheading:2407782-Cytokines, pubmed-meshheading:2407782-Epithelial Cells, pubmed-meshheading:2407782-Extracellular Matrix, pubmed-meshheading:2407782-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:2407782-Growth Substances, pubmed-meshheading:2407782-Interleukin-2, pubmed-meshheading:2407782-Interleukin-3, pubmed-meshheading:2407782-Lectins, pubmed-meshheading:2407782-Male, pubmed-meshheading:2407782-Mesoderm, pubmed-meshheading:2407782-Mice, pubmed-meshheading:2407782-Mice, Inbred Strains, pubmed-meshheading:2407782-Peanut Agglutinin, pubmed-meshheading:2407782-Thymus Gland
pubmed:year
1990
pubmed:articleTitle
Analysis of thymic stromal cell subpopulations grown in vitro on extracellular matrix in defined medium. II. Cytokine activities in murine thymic epithelial and mesenchymal cell culture supernatants.
pubmed:affiliation
Department of Life Science, Bar-Ilan University, Ramat-Gan, Israel.
pubmed:publicationType
Journal Article