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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1990-4-9
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pubmed:abstractText |
A 138-kDa nuclear protein was identified from HeLa cell extracts as a factor which binds to a previously described 20-base pair cis element located in the intron I of the c-myc gene. This myc intron factor (MIF) binds to the wild type c-myc sequence but does not bind under similar conditions to c-myc from Burkitt's lymphoma which contain point mutations in this binding region. We have demonstrated that the 138-kDa MIF is a phosphoprotein and that treatment of the purified MIF with potato acid phosphatase abolished binding to its 20-base pair c-myc recognition sequence; binding activity was protected by inclusion of phosphatase inhibitors. These results suggest that phosphorylation is required for the specific DNA-MIF interaction in vitro and that the phosphorylation state of MIF may be an important factor in controlling c-myc expression in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
265
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4547-51
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2407738-Acid Phosphatase,
pubmed-meshheading:2407738-Base Sequence,
pubmed-meshheading:2407738-Binding Sites,
pubmed-meshheading:2407738-Cell Nucleus,
pubmed-meshheading:2407738-DNA,
pubmed-meshheading:2407738-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2407738-HeLa Cells,
pubmed-meshheading:2407738-Humans,
pubmed-meshheading:2407738-Introns,
pubmed-meshheading:2407738-Molecular Sequence Data,
pubmed-meshheading:2407738-Mutation,
pubmed-meshheading:2407738-Nuclear Proteins,
pubmed-meshheading:2407738-Oligonucleotide Probes,
pubmed-meshheading:2407738-Phosphoproteins,
pubmed-meshheading:2407738-Phosphorylation,
pubmed-meshheading:2407738-Proto-Oncogene Proteins,
pubmed-meshheading:2407738-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:2407738-Regulatory Sequences, Nucleic Acid
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pubmed:year |
1990
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pubmed:articleTitle |
Phosphorylation-dependent binding of a 138-kDa myc intron factor to a regulatory element in the first intron of the c-myc gene.
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pubmed:affiliation |
Medicine Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article
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