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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1990-3-26
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pubmed:abstractText |
Saccharomyces cerevisiae RNA polymerase II conditional mutants that selectively disrupt the synthesis of specific mRNAs were isolated. At the permissive temperature, several of the mutants were inositol auxotrophs as a result of inadequate induction of INO1 transcription. The transcriptional defects exhibited by one of these Ino- mutants (rpb2-2) were further investigated. The induction of GAL10 and HIS4 transcription in rpb2-2 strains was similar to that of wild-type strains, in contrast to the lack of induction of INO1 transcription. When shifted to the nonpermissive temperature, cells containing rpb2-2 continued to accumulate some mRNAs but not others. Together, these results indicate that transcription of specific genes can be disrupted by RNA polymerase II mutations. The rpb2-2 allele alters an amino acid residue that occurs in a highly conserved segment of the RPB2 protein and that is shared by homologous subunits in other species.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-2406567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-2537149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-2667136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-2674672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-2685562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-2828185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-2894251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-2937059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3025587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3031449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3116266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3299050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3319781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3323810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3327472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3528751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3528752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3530495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3547406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3815519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-387258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3889913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3889924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3893883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-3896517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-6266829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-6276569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-6326108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-6356359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-6361500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-6417125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-6427038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-6777048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-6785043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-6799516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-6819424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-8608933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2406558-94253
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1010-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2406558-Amino Acid Sequence,
pubmed-meshheading:2406558-Blotting, Northern,
pubmed-meshheading:2406558-Gene Expression Regulation, Fungal,
pubmed-meshheading:2406558-Genes, Fungal,
pubmed-meshheading:2406558-Molecular Sequence Data,
pubmed-meshheading:2406558-Mutation,
pubmed-meshheading:2406558-RNA, Fungal,
pubmed-meshheading:2406558-RNA, Messenger,
pubmed-meshheading:2406558-RNA Polymerase II,
pubmed-meshheading:2406558-Saccharomyces cerevisiae,
pubmed-meshheading:2406558-Transcription, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
RNA polymerase II mutants defective in transcription of a subset of genes.
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pubmed:affiliation |
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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