Linked Life Data

2406250

Source:http://linkedlifedata.com/resource/pubmed/id/2406250

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1990-3-23
pubmed:databankReference
pubmed:abstractText
The nonhistone chromosomal protein NHP6 from Saccharomyces cerevisiae has been previously isolated and its amino-terminal sequence determined. In this report, synthetic oligonucleotides, designed from the limited NHP6 amino acid sequence, were used as hybridization probes to clone the NHP6A gene from a yeast genomic library. Low stringency Southern blot analysis showed that there was a second gene homologous to NHP6A. This gene, NHP6B, was also cloned and sequenced. Nucleotide sequence analysis revealed that NHP6B has six extra amino acids at its amino terminus, but that NHP6A and NHP6B match at 87% of the rest of their sequences. S1 nuclease analysis was used to show that both genes are transcribed; the major transcription start sites lie 30 bases before the first ATG codon. Interestingly, the approximately 11-kDa NHP6A and NHP6B proteins are homologous to the middle segment of the 27-kDa chromatin-associated high mobility group protein 1 from calf; NHP6A and NHP6B each have over 40% identity with this part of high mobility group protein 1. Possible functions for the NHP6 proteins are discussed in light of this homology.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
265
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3234-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2406250-Amino Acid Sequence, pubmed-meshheading:2406250-Animals, pubmed-meshheading:2406250-Base Sequence, pubmed-meshheading:2406250-Blotting, Southern, pubmed-meshheading:2406250-Cattle, pubmed-meshheading:2406250-Chromosomal Proteins, Non-Histone, pubmed-meshheading:2406250-DNA, Fungal, pubmed-meshheading:2406250-Genes, Fungal, pubmed-meshheading:2406250-High Mobility Group Proteins, pubmed-meshheading:2406250-Molecular Sequence Data, pubmed-meshheading:2406250-Multigene Family, pubmed-meshheading:2406250-Nucleic Acid Hybridization, pubmed-meshheading:2406250-Oligonucleotide Probes, pubmed-meshheading:2406250-RNA, Fungal, pubmed-meshheading:2406250-Restriction Mapping, pubmed-meshheading:2406250-Saccharomyces cerevisiae, pubmed-meshheading:2406250-Sequence Homology, Nucleic Acid
pubmed:year
1990
pubmed:articleTitle
Duplicated NHP6 genes of Saccharomyces cerevisiae encode proteins homologous to bovine high mobility group protein 1.
pubmed:affiliation
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.