Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-2-8
pubmed:abstractText
In Escherichia coli, sn-glycerol-3-phosphate can be oxidized by two different flavo-dehydrogenases, an anaerobic enzyme encoded by the glpACB operon and an aerobic enzyme encoded by the glpD operon. These two operons belong to the glp regulon specifying the utilization of glycerol, sn-glycerol-3-phosphate, and glycerophosphodiesters. In glpR mutant cells grown under conditions of low catabolite repression, the glpA operon is best expressed anaerobically with fumarate as the exogenous electron acceptor, whereas the glpD operon is best expressed aerobically. Increased anaerobic expression of glpA is dependent on the fnr product, a pleiotropic activator of genes involved in anaerobic respiration. In this study we found that the expression of a glpA1(Oxr) (oxygen-resistant) mutant operon, selected for increased aerobic expression, became less dependent on the FNR protein but more dependent on the cyclic AMP-catabolite gene activator protein complex mediating catabolite repression. Despite the increased aerobic expression of glpA1(Oxr), a twofold aerobic repressibility persisted. Moreover, anaerobic repression by nitrate respiration remained normal. Thus, there seems to exist a redox control apart from the FNR-mediated one. We also showed that the anaerobic repression of the glpD operon was fully relieved by mutations in either arcA (encoding a presumptive DNA recognition protein) or arcB (encoding a presumptive redox sensor protein). The arc system is known to mediate pleiotropic control of genes of aerobic function.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-2644240, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-2838728, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-2850440, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-2964639, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3012272, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3027032, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3035558, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3045516, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3049531, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3286606, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3298218, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3301812, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3323848, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3329696, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3536878, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-374371, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-3882660, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-4289962, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-4580569, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-4945192, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-6304089, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-6321507, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-6343617, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-6363389, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-6387427, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-7047497, http://linkedlifedata.com/resource/pubmed/commentcorrection/2403539-796407
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
172
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-84
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Multiple regulatory elements for the glpA operon encoding anaerobic glycerol-3-phosphate dehydrogenase and the glpD operon encoding aerobic glycerol-3-phosphate dehydrogenase in Escherichia coli: further characterization of respiratory control.
pubmed:affiliation
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.