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rdf:type |
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lifeskim:mentions |
umls-concept:C0014442,
umls-concept:C0017262,
umls-concept:C0032005,
umls-concept:C0035696,
umls-concept:C0185117,
umls-concept:C0204727,
umls-concept:C0205245,
umls-concept:C0205409,
umls-concept:C0205419,
umls-concept:C1519623,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
1990-10-24
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pubmed:databankReference |
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pubmed:abstractText |
We demonstrate that, in rat pituitary, peptidylglycine alpha-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
319-23
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2401356-Amino Acid Sequence,
pubmed-meshheading:2401356-Animals,
pubmed-meshheading:2401356-Base Sequence,
pubmed-meshheading:2401356-Blotting, Southern,
pubmed-meshheading:2401356-Cell Line,
pubmed-meshheading:2401356-Cell Membrane,
pubmed-meshheading:2401356-DNA,
pubmed-meshheading:2401356-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:2401356-Gene Library,
pubmed-meshheading:2401356-Genetic Variation,
pubmed-meshheading:2401356-Kinetics,
pubmed-meshheading:2401356-Mixed Function Oxygenases,
pubmed-meshheading:2401356-Molecular Sequence Data,
pubmed-meshheading:2401356-Multienzyme Complexes,
pubmed-meshheading:2401356-Pituitary Gland,
pubmed-meshheading:2401356-Plasmids,
pubmed-meshheading:2401356-RNA, Messenger,
pubmed-meshheading:2401356-Rats,
pubmed-meshheading:2401356-Rats, Inbred Strains,
pubmed-meshheading:2401356-Restriction Mapping,
pubmed-meshheading:2401356-Transfection
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pubmed:year |
1990
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pubmed:articleTitle |
Isolation and functional expression of pituitary peptidylglycine alpha-amidating enzyme mRNA. A variant lacking the transmembrane domain.
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pubmed:affiliation |
Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.
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pubmed:publicationType |
Journal Article
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