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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1990-10-18
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pubmed:abstractText |
Hepatitis delta virus (HDV) RNA subfragments undergo self-cleavage at varying efficiencies. We have developed a procedure of using repeated cycles of heat denaturation and renaturation of RNA to achieve a high efficiency of cleavage. This effect can also be achieved by gradual denaturation of RNA with heat or formamide. These results suggest that only a subpopulation of the catalytic RNA molecules assumes the active conformation required for self-cleavage. This procedure could be of general use for detecting catalytic RNA activities.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-16593780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-17833317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-2414014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-2429192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-2433049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-2436805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-2441261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-2474803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-2492677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-2648383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-3184270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-3367426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-3627276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-3762705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2398903-6163133
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5575-9
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:2398903-Catalysis,
pubmed-meshheading:2398903-Formamides,
pubmed-meshheading:2398903-Hepatitis Delta Virus,
pubmed-meshheading:2398903-Hydrogen-Ion Concentration,
pubmed-meshheading:2398903-Magnesium,
pubmed-meshheading:2398903-Nucleic Acid Conformation,
pubmed-meshheading:2398903-Nucleic Acid Denaturation,
pubmed-meshheading:2398903-Nucleic Acid Precursors,
pubmed-meshheading:2398903-Nucleic Acid Renaturation,
pubmed-meshheading:2398903-RNA, Viral,
pubmed-meshheading:2398903-Temperature
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pubmed:year |
1990
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pubmed:articleTitle |
RNA conformational requirements of self-cleavage of hepatitis delta virus RNA.
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pubmed:affiliation |
Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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