Linked Life Data

2396164

Source:http://linkedlifedata.com/resource/pubmed/id/2396164

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-10-11
pubmed:abstractText
Cultured endothelial cells are currently used as a model for the study of the interaction of platelets with the vascular wall under flow conditions. Little is known about the influence of cell-culture conditions on the preservation of the original characteristics of primary tissues. In our study, the reactivity of platelets towards extracellular matrices produced by endothelial cells from different passage numbers was morphometrically evaluated. The presence and distribution of the extracellular von Willebrand factor was analyzed by immunofluorescent techniques. Morphology of endothelial cells in culture varied dramatically along the culture and polymorphism increased clearly at fourth or fifth passages. Extracellular matrix thrombogenicity decreased significantly from the fifth passage number. Simultaneously, von Willebrand factor distribution in ECM changed its typical pattern. Our results confirm that the extracellular matrix produced by human umbilical vein derived-endothelial cells constitutes a good model for the study of platelet adhesion onto vascular subendothelium. The limited viability of ECs and the variation in the thrombogenic properties of ECM formed after multiple passages must be taken into account when these cells or their products are used in experimental research.
pubmed:language
spa
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0036-4355
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
173-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
[Variations in the thrombogenic properties of the extracellular matrix produced by endothelial cells in consecutive passages].
pubmed:affiliation
Servicio de Hemoterapia y Hemostasia, Hospital Clínico y Provincial, Universidad de Barcelona, España.
pubmed:publicationType
Journal Article, Comparative Study, English Abstract, Research Support, Non-U.S. Gov't