Linked Life Data

2394710

Source:http://linkedlifedata.com/resource/pubmed/id/2394710

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
1990-10-9
pubmed:abstractText
We have utilized fura-2 and a Ca2+ surrogate, Mn2+, to assess the mechanism of Ca2+ entry involved in the refill of the internal agonist-sensitive Ca2+ pool in parotid acini. Both the muscarinic agonist, carbachol, and the alpha-adrenergic agonist, epinephrine, stimulate Mn2+ entry into dispersed parotid acini, which is detected as an augmentation in fura-2 fluorescence quench rate. The rate of Mn2+ entry into parotid acini, depleted of internal agonist-sensitive Ca2+ pools by prolonged carbachol stimulation in a nominally Ca2(+)-free medium, is not significantly changed by the addition of the muscarinic antagonist, atropine, but is significantly attenuated when these internal pools are allowed to either partially or totally reload with Ca2+. Also, we provide evidence which suggests that under conditions which promote refill, Mn2+ appears to directly enter the cytosol from the extracellular medium and is not accumulated into an internal Ca2+ pool either directly from the medium or via a cytosolic route. Thus, we suggest that during refill, Ca2+ enters into the cytosol prior to its recruitment into the internal agonist-sensitive Ca2+ pool and in turn, the magnitude of this entry is modulated by the refill status of this pool.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
265
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15010-4
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Refill status of the agonist-sensitive Ca2+ pool regulates Mn2+ influx into parotid acini.
pubmed:affiliation
Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
pubmed:publicationType
Journal Article