Linked Life Data

2393907

Source:http://linkedlifedata.com/resource/pubmed/id/2393907

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1990-10-5
pubmed:abstractText
A simple dye-coupling assay has been developed to study gap junctional communication using a uterine cell line. The new dye-coupling assay was found to be of comparable sensitivity to an autoradiographic assay for transfer of radiolabelled nucleotide. To investigate the importance of protein synthesis in the establishment of dye-coupling under the conditions of this assay, cultures were treated with actinomycin-D and cycloheximide to inhibit transcription and translation, respectively. Inhibition of transcription had no significant effect on dye-coupling. The protein synthesis inhibitor, cycloheximide, also had no significant effect on dye-coupling at concentrations up to 1 uM, even though protein synthesis was inhibited to 19% of control.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0309-1651
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
613-28
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
A simple dye-coupling assay for evaluating gap junctional communication: the importance of transcription and translation on the establishment of dye-coupling.
pubmed:affiliation
Department of Environmental and Industrial Health, University of Michigan School of Public Health, Ann Arbor 48109-2029.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't